Leukaemic stem cells in AML: where are we now? An update on recent findings and detection

BJH - volume 11, issue 6, october 2020

B. Depreter PhD, PharmD, B. De Moerloose MD, PhD, J. Philippé MD, PhD, T. Lammens PhD


Ample evidence was provided these past decades that leukaemic stem cells (LSC) play a role in the outcome of adult and paediatric acute myeloid leukaemia (AML) patients. Although it is generally accepted that the CD34+/ CD38- compartment is most LSC-enriched, novel data have emerged illustrating a distinct biology between CD34+ and CD34- AML. In this review, we discuss the main LSC phenotypes in CD34+ and CD34- AML , as they are of utmost importance for the development of broadly applicable LSC-targeted strategies. The leukaemia-initiating capacity of these cells upon xenografting is still considered to be the gold standard for LSC detection. However, more feasible techniques have been researched to allow the implementation of LSC measurements into clinical practice. Here, we summarise the current state-of-the-art methodologies using flow cytometry and molecular detection, and emphasise their relevance in terms of prognosis and targeted drug therapy.

(BELG J HEMATOL 2020;11(6):246-52)

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Joint BSAC-NVC Congress 2019: A colourful world

BJH - volume 11, issue 4, june 2020

N. Cools PhD, B. Depreter PhD, PharmD, V.H.J. van der Velden PhD


In November 2019, the Belgian Society for the Advancement of Cytometry (BSAC) and the Dutch Society for Cytometry (NVC) jointly organised a two-day international congress on developments in (flow) cytometry. With over 150 participants, the congress was well attended and received. Here we briefly discuss the most important highlights from this congress.

(BELG J HEMATOL 2020;11(4):185–9)

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P76 PrimeFlow™ RNA Assay: a novel technique for single-cell expression analysis in heterogeneous cell populations

BJH - volume 8, issue Abstract Book BHS, february 2017

B. Depreter PhD, PharmD, M. Meul , B. Denys MD, B. De Moerloose MD, PhD, E. Terras , K. Vandepoele PhD, J. Philippé MD, PhD, T. Lammens PhD

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P1.06 Distinguishing leukemic B-lymphoblasts from normal B-cell precursors using cut-off values for expression of CD24, CD38, CD58 and CD66c

BJH - volume 7, issue Abstract Book BHS, january 2016

B. Depreter PhD, PharmD, J. Philippé MD, PhD, B. Denys MD

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Clotting time or Rosner index for mixing test interpretation makes a difference in the overall lupus anticoagulant test result

BJH - volume 6, issue Abstract Book BSTH, november 2015

B. Depreter PhD, PharmD, K. Devreese

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Biclonal biphenotypic B-cell lymphocytosis: a CLL in disguise?

BJH - volume 5, issue 4, december 2014

B. Depreter PhD, PharmD, E. Dumoulin PharmD, J. Billiet MD, B. Cauwelier MD, PhD, B. Maes MD, PhD, C. Matthys MD, J. Van Droogenbroeck MD, F. Nollet PhD, MSc, J. Emmerechts MD, PhD


We report a rare case of biclonal biphenotypic B-cell lymphocytosis with chronic lymphocytic leukaemia phenotype. Clonality was initially misjudged by immunophenotyping because of its polyphenotypic pattern. Polymerase chain reaction analysis revealed clonality of the immunoglobulin heavy chain and kappa light chain gene rearrangement, indicating the presence of a monoclonal B-lymphocyte population. Immunophenotyping was repeated after cell sorting and revealed two CD5+CD19+ populations with different light chain restriction. Different genetic abnormalities for both clones, as evidenced by fluorescence in situ hybridisation and hypermutation analysis, support the diagnosis of two independently originated but co-existing B-cell clones. This case illustrates the importance of using multiple techniques in the diagnostic work-up of haematological malignancies.

(BELG J HEMATOL 2014;5(4):143–7)

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P2.02 The value of SOX11 overexpression in the differential diagnosis of mantle cell lymphoma

BJH - volume 5, issue Abstract Book BHS, january 2014

B. Depreter PhD, PharmD, F. Nollet PhD, MSc, S. Vermeire , B. Cauwelier MD, PhD, A. Van Hoof MD, PhD, J. Billiet MD

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